Canola cultivar 15rh0613

ABSTRACT

This invention relates to a canola hybrid cultivar designated 15RH0613 that includes plants, DNA, and seeds of canola 15RH0613. Methods for producing canola plants, such as canola plant varieties, hybrid canola plants, or other canola plants, as by crossing canola 15RH0613 with itself or any different canola plant are also part of this invention as are the resultant canola plants including the plant parts and seeds.

This application claims the benefit of U.S. Provisional PatentApplication, Ser. No. 62/383,740, filed, Sep. 6, 2016, entitled “CANOLACULTIVAR 15RH0613”, which application is hereby incorporated byreference in its entirety.

FIELD OF THE INVENTION

This invention relates to a canola hybrid cultivar designated 15RH0613that includes plants, DNA, and seeds of canola 15RH0613. Methods forproducing canola plants, such as canola plant varieties, hybrid canolaplants, or other canola plants, as by crossing canola 15RH0613 withitself or any different canola plant are also part of this invention asare the resultant canola plants including the plant parts and seeds.This invention further relates to methods for producing 15RH0613 derivedcanola plants and to methods for regenerating such plants from tissuecultures of regenerable cells as well as the plants obtained therefrom.Methods for producing a canola plant from 15RH0613 containing in itsgenetic material one or more transgenes and to the transgenic canolaplants produced by that method are also part of this invention.

BACKGROUND OF THE INVENTION

“Canola”, refers to a particular class of rapeseed (Brassica napusoleifera annua) having: (i) a seed oil that contains less than 2% erucicacid, and (ii) an oil-free meal that contains less than 30 micromolesaliphatic glucosinolates per gram of meal. Canola seed can be extractedor pressed for cooking oil and the residual meal is used as an organicfertilizer and as a high-protein animal feed supplement. Industrial usesof canola include biodiesel and plastic feedstocks.

Farmers in Canada began producing canola oil in 1968. Early canolacultivars were known as single zero cultivars because their oilcontained 5% or less erucic acid, but the glucosinolates content wasstill higher than desired. In 1974, the first licensed double zerocultivars (low erucic acid and low glucosinolates) were grown. Allcurrent canola cultivars are double zero cultivars.

Because the fatty acid profile of canola oil is generally viewed as“healthy”, its use is rising steadily both as an oil for cooking and asan ingredient in processed foods. It is generally lower in saturatedfatty acids and high in monounsaturated fatty acids than other seedoils. In addition, many people prefer the light color and mild taste ofcanola oil over other oils that contain monounsaturated fatty acids.

The goal of a canola breeder is to develop new, unique, and superiorcanola cultivars having improved desirable traits. Improved performanceis manifested in many ways. Higher yields of canola plants contribute tohigher seed production per acre, a more profitable agriculture and alower cost of products for the consumer. Improved oil profiles andquality of resulting oil is an important factor in the development ofnew canola cultivars. Adapting canola plants to a wider range ofproduction areas achieves improved yield and vegetative growth. Improvedplant uniformity enhances the farmer's ability to mechanically harvestcanola. Improved nutritional quality increases its value in food andfeed.

The development of new cultivars in a canola plant breeding programinvolves numerous steps, including: (1) selection of parent canolaplants for the initial breeding crosses; (2) producing and selectinginbred breeding lines and cultivars by either the doubled-haploid methodor repeated generations of selfing individual plants; (3) producing andselecting hybrid cultivars by crossing a selected inbred male-sterileline with an unrelated inbred restorer line to produce the F1 hybridprogeny having restored vigor; and (4) thoroughly testing these advancedinbreds and hybrids compared to appropriate standards for three or moreyears in environments representative of the commercial target areas.

Development and selection of new canola parental lines, the crossing ofthese parental lines, and selection of superior hybrid progeny are vitalto maintaining a canola breeding program. The F1 hybrid canola seed isproduced by manual crosses between selected male-fertile parents or byusing male-sterility systems. These hybrids are selected for certainsingle-gene or multiple gene traits. Additional data on parental lines,as well as the phenotype of the hybrid, influence the breeder's decisionwhether to continue with the specific hybrid cross.

The method of doubled-haploid breeding consists of donor selection,microspore culture and chromosome doubling, embryo cold stress, plantletregeneration, ploidy analysis, and self-pollination to produce seed ofdoubled-haploid lines. The advantage of the doubled-haploid method isthat the time to develop a new completely homozygous and homogeneouscultivar can be reduced by 3 years compared to the conventionalinbreeding method of multiple generations of self-pollination.

These processes, which lead to the final step of marketing anddistribution of a cultivar, usually take from 8 to 12 years from thetime the parental cross is made. Therefore, development of new canolainbred and hybrid cultivars is a slow, costly process that requires theresources and expertise of plant breeders and numerous otherspecialists.

It is nearly impossible for two canola breeders to independently developgenetically-identical canola inbreds or hybrids expressing all the sametrait characteristics. The cultivars that are developed cannot bepredicted because the breeder's selection occurs in unique environments,with no control over meiotic genetic recombination (using conventionalbreeding procedures), and with millions of different possible geneticcombinations possible. A breeder of ordinary skill in the art cannotpredict the final resulting lines he/she develops, except possibly in avery gross and general fashion. It is unlikely a breeder could producethe same cultivar twice by using the exact same original parents and thesame selection techniques.

Canola cultivars and other sources of canola germplasm are thefoundation material for all canola breeding programs. Despite theexistence and availability of numerous canola cultivars and other sourcegermplasm, a need still exists for the development of improved germplasmto improve and maximize yield and oil quality.

The foregoing examples of the related art and limitations relatedtherewith are intended to be illustrative and not exclusive. Otherlimitations of the related art will become apparent to those of skill inthe art upon a reading of the specification.

SUMMARY OF THE INVENTION

According to the invention, there is provided a novel canola cultivardesignated 15RH0613. This invention thus relates to the seeds of canola15RH0613, to the plants, or plant parts, of canola 15RH0613 and tomethods for producing a canola plant produced by crossing the canola15RH0613 with itself or another canola cultivar, and the creation ofvariants by mutagenesis or transformation of canola 15RH0613.

Thus, any such methods using the canola 15RH0613 are part of thisinvention: selfing, backcrossing, hybrid production, crossing topopulations, and the like. All plants produced using canola 15RH0613 asa parent, are within the scope of this invention. Advantageously, thecanola 15RH0613 could be used in crosses with other, different, canolaplants to produce first generation (F1) canola hybrid seeds and plantswith superior characteristics.

In another aspect, the present invention provides for single or multiplegene-converted plants of canola 15RH0613. The transferred gene(s) maypreferably have dominant or recessive allele(s). Preferably, thetransferred gene(s) will confer such traits as herbicide resistance,insect resistance; resistance to bacterial, fungal, or viral disease;male fertility, male sterility, enhanced nutritional quality, orindustrial usage. The gene may be a naturally occurring canola gene or atransgene introduced through genetic engineering techniques.

In another aspect, the present invention provides regenerable cells foruse in tissue culture of canola 15RH0613. The tissue culture willpreferably be capable of regenerating plants having the physiologicaland morphological characteristics of the foregoing canola plant, and ofregenerating plants having substantially the same genotype as theforegoing canola plant. Preferably, the regenerable cells in such tissuecultures will be embryos, protoplasts, meristematic cells, callus,pollen, leaves, anthers, roots, root tips, flowers, seeds, pods orstems. Still further, the present invention provides canola plantsregenerated from the tissue cultures of the invention.

In another aspect, the present invention provides a method ofintroducing a desired trait into canola 15RH0613 wherein the methodcomprises: crossing a 15RH0613 plant with a plant of a different canolagenotype that comprises a desired trait to produce F1 progeny plants,wherein the desired trait is selected from the group consisting of malesterility, herbicide resistance, insect resistance, and resistance tobacterial disease, fungal disease or viral disease; selecting one ormore progeny plants that have the desired trait to produce selectedprogeny plants; crossing the selected progeny plants with the 15RH0613plants to produce backcross progeny plants; selecting for backcrossprogeny plants that have the desired trait and physiological andmorphological characteristics of canola 15RH0613 to produce selectedbackcross progeny plants; and repeating these steps to produce selectedfirst or higher backcross progeny plants that comprise the desired traitand all of the physiological and morphological characteristics of canola15RH0613. Included in this aspect of the invention is the plant producedby the method wherein the plant has the desired trait and all of thephysiological and morphological characteristics of canola 15RH0613.

In addition to the exemplary aspects and embodiments described above,further aspects and embodiments will become apparent by study of thefollowing descriptions.

DETAILED DESCRIPTION OF THE INVENTION

In the description and examples that follow, a number of terms are used.To provide a clear and consistent understanding of the specification andclaims, including the scope to be given such terms, the followingdefinitions are provided.

Definitions of Plant Characteristics

Backcrossing: A process in which a breeder repeatedly crosses hybridprogeny back to one of the parents; for example, a first generationhybrid F1 crossed back to one of the parental genotypes of the F1hybrid.

Cultivar: A plant genotype that has been intentionally bred or selectedto be genetically distinct, uniform, and stable, and is maintainedthrough cultivation or other propagation.

Days to Flowering: The number of days from planting to the stage when50% of the plants show one or more open flowers.

Days to Bolting: The number of days from planting to the appearance ofan elongating vegetative stem with a floral bud.

Days to Last Flower: The number of days from planting to when all of theflowers on a plant have opened.

Glyphosate Herbicide Resistance: Resistance of a plant to the action ofglyphosate; conferred in crops by genetic transformation of the cropplant using a 5-enol-pyruvylshikimate-3-phosphate synthase (EPSPS) genethat is insensitive to the effect of glyphosate, or a bacterialglyphosate oxidoreductase (GOX) gene that cleaves the nitrogen-carbonbond in glyphosate yielding aminomethylphosphonic acid.

Herbicide Resistance: When a plant has negligible effect from contactwith an herbicide because the plant does not take up the herbicide orsequesters the herbicide in a manner that renders it harmless.

Herbicide Tolerance: When a plant has negligible effect from contactwith an herbicide because the plant metabolically detoxifies theherbicide.

Hybrid: A cultivar or plant-breeding progeny based upon the controlledcross-pollination between or among distinct parent lines, so that theresulting seed inherits its genetic composition from those parent lines.Seed for a particular hybrid can be repeatedly and predictably producedwhen repeatedly making controlled cross-pollinations from the samestable female and male parent genotypes.

Inbred: A relatively stable plant genotype resulting from doubledhaploids, successive generations of controlled self-pollination,successive generations of controlled backcrossing to a recurrent parent,or other method to develop homozygosity.

Linoleic Acid: means a (C18:2) fatty acid.

Linolenic Acid: means a (C18:3) fatty acid.

Oleic Acid: means a (C18:1) fatty acid.

Palmitic Acid: means a (C16:0) fatty acid.

Seed Weight: The weight in grams of 1000 seeds at maturity at 5% to 6%moisture.

Stearic Acid: means a (C18:0) fatty acid.

Total Saturates: means the combination of the percentages of thefollowing fatty acids which may be present in canola oils. TotalSaturates refers to the total of myristic acid (C14:0), palmitic acid(C16:0), stearic acid (C18:0), arachidic acid (C20:0), behenic acid(C22:0), and lignoceric acid (C24:0).

Yield: means quantity of grain produced in grams per plant.

Description of 15RH0613

Canola 15RH0613 is a hybrid cultivar produced from a cross betweenproprietary Cargill canola inbreds 14CA3082.30 and 14RR5004.72.0075.

Canola 15RH0613 has resistance to the herbicide glyphosate. Fields ofcanola are subject to a wide variety of weedy species, includingvolunteer plants from a previous crop, which can affect the oil profileof the harvested crop. Use of a glyphosate herbicide in a canola hybridcrop that has glyphosate herbicide resistance provides broad-spectrumcontrol of weedy species in that crop. The glyphosate herbicideresistance in canola 15RH0613 provides an effective means to maintainthe integrity of the desired harvested oil profile of a hybrid cropderived from canola 15RH0613 by reducing the contamination of seed fromvolunteer plants and weedy species that are not easily separated fromthe harvested canola grain within commercial production.

The cultivar 15RH0613 has shown uniformity and stability ofcharacteristics over multiple generations of testing. The productionparents have been self-pollinated and can be increased through multiplegenerations with careful attention to uniformity of plant type for theparent lines and consequently for 15RH0613.

The present invention relates to a canola plant that expressessubstantially all of the physiological and morphological characteristicsof cultivar 15RH0613. Any plants produced from cultivar 15RH0613 arecontemplated by the present invention and are, therefore, within thescope of this invention. A description of morphological and othercharacteristics of cultivar 15RH0613 are presented in Table 1.

TABLE 1 Morphological and Other Characteristics of Canola Brassica napus15RH0613 Characteristic Value (variance) Season Type (Spring, Winter)Spring Type of Cultivar Hybrid Days to Bolting 44.61 (8.14) Days toFlower 55.39 (10.64) Days to Last Flower 77.56 (15.49) Harvest Index0.09 (0.02) Yield (g) per plant 2.11 (0.52) Seed Weight 3.31 (0.37)

Canola oils produced from 15RH0613 are particularly useful for fryingapplications. The major challenges faced by fast food restaurants infrying involves the desire to have a good tasting oil that is alsostable to the intense high heat and moisture inherent in fryingconditions. Coupled with those challenges is the desire to have oilsthat are also reduced in saturated fatty acids. Fully saturated fats,either naturally occurring or produced through hydrogenation of otheroils have excellent stability, however, these oils are viewed negativelyby the public. The oils of 15RH0613 were developed to address the threepronged need for a frying oil to be stable, great tasting, and low intotal saturates. Stability is achieved by maintaining a low content oflinolenic acid (C18:3). This fatty acid is particularly susceptible tooxidation in the frying environment. Flavor is maintained by keeping ahigh content of linoleic acid (C18:2). Finally, the customer awarenessaround saturated fat is managed by having an oil with less than 5% totalsaturates. All three of these important parameters had never beenachieved in canola crop. Accordingly, oils of 15RH0613 can be usedproduce fried foods such as snack chips (e.g., corn or potato chips),french fries, or other quick serve foods.

In addition, canola hybrid 15RH0613 has exhibited a unique and valuablefatty acid profile in the oil extracted from it mature seeds grown infield trials at various locations. Oil characteristics are set out inTable II below.

TABLE II % C18:1 Cultivar (min, max) % C18:2 % C18:3 % Total Sats15RH0613 67.13 24.20 1.90 4.46 (62.2, 73.6) (18.0, 28.8) (1.4, 2.8)(3.8, 5.9)

15RH0613 and any cultivar derived from 15RH0613 as contemplated hereinmay have a C18:3 level in the canola seed or oil of between 1.5% and 3%.Additional embodiments have levels from i) 1.5% to 2.5%; ii) 1.65% to2.5%; or iii) 1.1% to 3.1%.

15RH0613 and any cultivar derived from 15RH0613 as contemplated hereinmay have a C18:2 linoleic acid level in the canola seed or oil ofgreater than 18% or 20%. Additional embodiments have levels from i) 21%to 28%; ii) 24% to 26%; iii) 21.1% to 28.8%; or iv) 18% to 30.6%.

15RH0613 and any cultivar derived from 15RH0613 as contemplated hereinmay have a C18:1 oleic acid level in the canola seed or oil of greaterthan 60% or 65%. Additional embodiments have levels from i) 60% to 70%;ii) 63% to 68%; or iii) 59.9% to 73.6%.

15RH0613 and any cultivar derived from 15RH0613 as contemplated hereinhave a total saturates level of less than 5%. Commodity canola oilscommonly used in industry and by consumers have a saturate levels ofbetween 6-8%. See, e.g., Bailey's Industrial Oil and Fat Products,Section 2.2, “Canola Oil” on pages 61-121 of Volume 2 (6th Edition,2005). Embodiments of the present invention have total saturates levelin the canola seed or oil of between 3.5% and 5%. Additional embodimentshave levels from i) 4% to 5%; ii) 4 to 4.5%; iii) 4.2% to 4.7%; and iv)3.8% to 5.9%.

The fatty acid composition of oil obtained from seed of Brassica plantscan be determined by methods well known in the art. Typically it can bedetermined by first crushing and extracting oil from seed samples (e.g.,bulk seed samples of 10 or more seeds). TAGs in the seed are hydrolyzedto produce free fatty acids, which then can be converted to fatty acidmethyl esters and analyzed using techniques known to the skilledartisan, e.g., gas-liquid chromatography (GLC) according to AOCSProcedure Ce 1-62. Near infrared (NIR) analysis can be performed onwhole seed according to AOCS Procedure Am-192 (revised 1999). Numericalvalues presented herein are a weight percentage. The percentage of aparticular fatty acid is described as a percentage of the total fattyacids in the sample as identified empirically.

This invention is also directed to methods for producing a canola plantby crossing a first parent canola plant with a second parent canolaplant, wherein the first or second canola plant is a canola plant from15RH0613. Further, both first and second parent canola plants may befrom 15RH0613. Therefore, any methods using 15RH0613 are part of thisinvention: selfing, backcrosses, hybrid breeding, and crosses topopulations. Any plants produced using 15RH0613 as parents are withinthe scope of this invention.

Tissue Culture of Canola

Further production of the 15RH0613 can occur by self-pollination or bytissue culture and regeneration. Tissue culture of various tissues ofcanola and regeneration of plants therefrom is known. For example, thepropagation of a canola cultivar by tissue culture is described in anyof the following but not limited to any of the following: Chuong et al.,“A Simple Culture Method for Brassica hypocotyls Protoplasts,” PlantCell Reports 4:4-6 (1985); Barsby, T. L., et al., “A Rapid and EfficientAlternative Procedure for the Regeneration of Plants from HypocotylProtoplasts of Brassica napus,” Plant Cell Reports (Spring, 1996);Kartha, K., et al., “In vitro Plant Formation from Stem Explants ofRape,” Physiol. Plant, 31:217-220 (1974); Narasimhulu, S., et al.,“Species Specific Shoot Regeneration Response of Cotyledonary Explantsof Brassicas,” Plant Cell Reports (Spring 1988); Swanson, E.,“Microspore Culture in Brassica,” Methods in Molecular Biology, Vol. 6,Chapter 17, p. 159 (1990).

Further reproduction of the variety can occur by tissue culture andregeneration. Tissue culture of various tissues of soybeans andregeneration of plants therefrom is well known and widely published. Forexample, reference may be had to Komatsuda, T. et al., “Genotype XSucrose Interactions for Somatic Embryogenesis in Soybeans,” Crop Sci.31:333-337 (1991); Stephens, P. A., et al., “Agronomic Evaluation ofTissue-Culture-Derived Soybean Plants,” Theor. Appl. Genet. (1991)82:633-635; Komatsuda, T. et al., “Maturation and Germination of SomaticEmbryos as Affected by Sucrose and Plant Growth Regulators in SoybeansGlycine gracilis Skvortz and Glycine max (L.) Merr.” Plant Cell, Tissueand Organ Culture, 28:103-113 (1992); Dhir, S. et al., “Regeneration ofFertile Plants from Protoplasts of Soybean (Glycine max L. Merr.);Genotypic Differences in Culture Response,” Plant Cell Reports (1992)11:285-289; Pandey, P. et al., “Plant Regeneration from Leaf andHypocotyl Explants of Glycine-wightii (W. and A.) VERDC. var.longicauda,” Japan J. Breed. 42:1-5 (1992); and Shetty, K., et al.,“Stimulation of In Vitro Shoot Organogenesis in Glycine max (Merrill.)by Allantoin and Amides,” Plant Science 81:245-251 (1992). Thedisclosures of U.S. Pat. No. 5,024,944 issued Jun. 18, 1991 to Collinset al., and U.S. Pat. No. 5,008,200 issued Apr. 16, 1991 to Ranch etal., are hereby incorporated herein in their entirety by reference.Thus, another aspect of this invention is to provide cells which upongrowth and differentiation produce canola plants having thephysiological and morphological characteristics of canola 15RH0613.

As used herein, the term “tissue culture” indicates a compositioncomprising isolated cells of the same or a different type or acollection of such cells organized into parts of a plant. Exemplarytypes of tissue cultures are protoplasts, calli, plant clumps, and plantcells that can generate tissue culture that are intact in plants orparts of plants, such as embryos, pollen, flowers, seeds, pods, leaves,stems, roots, root tips, anthers, and the like. Means for preparing andmaintaining plant tissue culture are well known in the art. By way ofexample, a tissue culture comprising organs has been used to produceregenerated plants. U.S. Pat. Nos. 5,959,185, 5,973,234 and 5,977,445,describe certain techniques, the disclosures of which are incorporatedherein by reference.

Single-Gene Converted (Conversion) Plants

When the term “canola plant” is used in the context of the presentinvention, this also includes any single-gene conversions of thatvariety. The term “single-gene converted plant” as used herein refers tothose canola plants that are developed by a plant breeding techniquecalled backcrossing, or via genetic engineering, wherein essentially allof the desired morphological and physiological characteristics of avariety are recovered in addition to the single gene transferred intothe variety via the backcrossing technique. Backcrossing methods can beused with the present invention to improve or introduce a characteristicinto the variety. The term “backcrossing” as used herein refers to therepeated crossing of a hybrid progeny back to the recurrent parent,i.e., backcrossing 1, 2, 3, 4, 5, 6, 7, 8 or more times to the recurrentparent. The parental canola plant that contributes the gene for thedesired characteristic is termed the “non-recurrent” or “donor parent.”This terminology refers to the fact that the non-recurrent parent isused one time in the backcross protocol and therefore does not recur.The parental canola plant to which the gene or genes from thenon-recurrent parent are transferred is known as the recurrent parent asit is used for several rounds in the backcrossing protocol (Poehlman &Sleper, 1994; Fehr, 1987). In a typical backcross protocol, the originalvariety of interest (recurrent parent) is crossed to a second variety(non-recurrent parent) that carries the single gene of interest to betransferred. The resulting progeny from this cross are then crossedagain to the recurrent parent and the process is repeated until a canolaplant is obtained wherein essentially all of the desired morphologicaland physiological characteristics of the recurrent parent are recoveredin the converted plant, in addition to the single transferred gene fromthe non-recurrent parent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalvariety. To accomplish this, a single gene of the recurrent variety ismodified or substituted with the desired gene from the non-recurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphological,constitution of the original variety. The choice of the particularnon-recurrent parent will depend on the purpose of the backcross. One ofthe major purposes is to add some commercially desirable, agronomicallyimportant trait to the plant. The exact backcrossing protocol willdepend on the characteristic or trait being altered to determine anappropriate testing protocol. Although backcrossing methods aresimplified when the characteristic being transferred is a dominantallele, a recessive allele may also be transferred. In this instance itmay be necessary to introduce a test of the progeny to determine if thedesired characteristic has been successfully transferred.

Many single-gene traits have been identified that are not regularlyselected for in the development of a new variety but that can beimproved by backcrossing techniques. Single gene traits may or may notbe transgenic, examples of these traits include but are not limited to,male sterility, herbicide resistance, resistance for bacterial, fungal,or viral disease, insect resistance, male fertility, enhancednutritional quality, industrial usage, yield stability and yieldenhancement. These genes are generally inherited through the nucleus.Several of these single-gene traits are described in U.S. Pat. Nos.5,959,185, 5,973,234 and 5,977,445, the disclosures of which arespecifically hereby incorporated by reference.

This invention also is directed to methods for producing a canola plantby crossing a first parent canola plant with a second parent canolaplant wherein the first or second parent canola plant is a canola plantof 15RH0613. Further, both first and second parent canola plants cancome from the canola 15RH0613. Thus, any such methods using the canolacultivar 15RH0613 are part of this invention: selfing, backcrosses,hybrid production, crosses to populations, and the like. All plantsproduced using canola 15RH0613 as a parent are within the scope of thisinvention, including those developed from varieties derived from canola15RH0613. Advantageously, the canola variety could be used in crosseswith other, different, canola plants to produce first generation (F1)canola hybrid seeds and plants with superior characteristics. Thecultivar of the invention can also be used for transformation whereexogenous genes are introduced and expressed by the cultivar of theinvention. Genetic variants created either through traditional breedingmethods using 15RH0613 or through transformation of 15RH0613 by any of anumber of protocols known to those of skill in the art are intended tobe within the scope of this invention.

While a number of exemplary aspects and embodiments have been discussedabove, those of skill in the art will recognize certain modifications,permutations, additions, and sub-combinations thereof. It is thereforeintended that the following appended claims and claims hereafterintroduced are interpreted to include all such modifications,permutations, additions and sub-combinations as are within their truespirit and scope.

All references, including publications, patents, and patentapplications, cited herein are hereby incorporated by reference to thesame extent as if each reference were individually and specificallyindicated to be incorporated by reference and were set forth in itsentirety herein. The references discussed herein are provided solely fortheir disclosure prior to the filing date of the present application.

DEPOSIT INFORMATION

A deposit of the Cargill Incorporated proprietary canola 15RH0613disclosed above and recited in the appended claims has been made withthe American Type Culture Collection (ATCC), 10801 University Boulevard,Manassas, Va. 20110. The date of deposit was Jun. 16, 2016 and thedeposit is intended to meet all of the requirements of 37 C.F.R.Sections 1.801-1.809. The ATCC accession number is PTA-12316. Thedeposit will be maintained in the depository for a period of 30 years,or 5 years after the last request, or for the effective life of thepatent, whichever is longer, and will be replaced as necessary duringthat period.

We claim:
 1. A seed of canola designated 15RH0613, wherein arepresentative sample of said seed was deposited under ATCC AccessionNo. PTA-12316.
 2. A canola plant, or a part thereof, derived from, orproduced by growing, the seed of claim
 1. 3. A method of introducing adesired trait into canola 15RH0613, wherein the method comprises: (a)crossing a 15RH0613 plant, wherein a representative sample of seed wasdeposited under ATCC Accession No. PTA-12316, with a plant of anothercanola cultivar that comprises a desired trait to produce F1 progenyplants, wherein the desired trait is selected from the group consistingof Total Saturates less than 5%, male sterility, herbicide resistance,insect resistance, and resistance to bacterial disease, fungal diseaseor viral disease; (b) selecting one or more progeny plants that have thedesired trait to produce selected progeny plants; (c) crossing theselected progeny plants with the 15RH0613 plants to produce backcrossprogeny plants; (d) selecting for backcross progeny plants that have thedesired trait and physiological and morphological characteristics ofcanola 15RH0613 to produce selected backcross progeny plants; and (e)repeating steps (c) and (d) three or more times to produce selectedfourth or higher backcross progeny plants that comprise the desiredtrait.
 4. The method of claim 3, wherein the plants further comprise aseed having an oil comprising (i) a total saturates of between 4% and5%; (ii) a linoleic acid content is from 21% to 28%; and (iii) alinolenic acid content is from 1.5% to 3.0%.
 5. The method of claim 3,wherein the plants further comprise herbicide resistance to an herbicideselected from the group consisting of imidazolinone, sulfonylurea,glyphosate, glufosinate, L-phosphinothricin, triazine, Clearfield,Dicamaba, 2,4-D, and benzonitrile.
 6. The method of claim 3, wherein theplants comprise all of the physiological and morphologicalcharacteristics of canola 15RH0613 as shown in Tables 1 and
 2. 7. Acanola plant produced by the method of claim 4, further comprising anoleic acid value of about 65%.
 8. The canola plant of claim 7, whereinthe desired trait further comprises herbicide resistance and theresistance is conferred to an herbicide selected from the groupconsisting of imidazolinone, sulfonylurea, glyphosate, glufosinate,L-phosphinothricin, triazine, Clearfield, Dicamaba, 2,4-D, andbenzonitrile.
 9. The canola plant of claim 8, wherein the desired traitfurther comprises insect resistance and the insect resistance isconferred by a transgene encoding a Bacillus thuringiensis endotoxin.10. The canola plant of claim 8, wherein the desired trait furthercomprises resistance to Blackleg, Fusarium wilt, or White Rust.
 11. Thecanola plant of claim 8, wherein the plant comprises all of thephysiological and morphological characteristics of canola 15RH0613, asshown in Tables 1 and
 2. 12. A method of modifying fatty acid metabolismor modifying carbohydrate metabolism of canola 15RH0613 wherein themethod comprises: (a) crossing a 15RH0613 plant, wherein arepresentative sample of seed was deposited under ATCC Accession No.PTA-12316, with a plant of another canola cultivar to produce F1 progenyplants that comprise a nucleic acid molecule encoding an enzyme selectedfrom the group consisting of phytase, fructosyltransferase,levansucrase, alpha-amylase, invertase and starch branching enzyme orencoding an antisense of stearyl-ACP desaturase; (b) selecting one ormore progeny plants that have said nucleic acid molecule to produceselected progeny plants; (c) crossing the selected progeny plants withthe 15RH0613 plants to produce backcross progeny plants; (d) selectingfor backcross progeny plants that have said nucleic acid molecule andphysiological and morphological characteristics of canola 15RH0613 toproduce selected backcross progeny plants; and (e) repeating steps (c)and (d) three or more times to produce selected fourth or higherbackcross progeny plants that comprise said nucleic acid molecule andhave a total saturates level of less than 5%, an oleic acid value ofabout 65%, and an a-linolenic acid value of less than about 3%.
 13. Themethod of claim 12, wherein the plants further comprise a seed having anoil comprising (i) a total saturates of between 4% and 5%; (ii) alinoleic acid content is from 21% to 28%; and (iii) a linolenic acidcontent is from 1.5% to 3.0%
 14. The method of claim 13, wherein theplants further comprise resistance to Blackleg (Leptosphaeria maculans),Fusarium wilt, or White Rust.
 15. The method of claim 13, wherein theplants further comprise herbicide resistance to an herbicide selectedfrom the group consisting of imidazolinone, sulfonylurea, glyphosate,glufosinate, L-phosphinothricin, triazine, Clearfield, Dicamaba, 2,4-D,and benzonitrile.
 16. The method of claim 13, wherein the plantscomprise all of the physiological and morphological characteristics ofcanola 15RH0613 as shown in Tables 1 and
 2. 17. A canola plant producedby the method of claim 13, wherein the plant comprises the nucleic acidmolecule and has an oleic acid value of about 65%.
 18. A canola plantproduced by the method of claim 13, wherein the plants comprise all ofthe physiological and morphological characteristics of canola 15RH0613as shown in Tables 1 and 2.